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primary antibody rabbit pab anti human tem8  (Bioss)


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    Bioss primary antibody rabbit pab anti human tem8
    Primary Antibody Rabbit Pab Anti Human Tem8, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+rabbit+pab+anti+human+tem8/TEM8+Polyclonal+Antibody/pmc12596184__sfaf301_supplemental_file-26-35-43
    Average 90 stars, based on 2 article reviews
    primary antibody rabbit pab anti human tem8 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Urinary endotrophin as a biomarker for T cell–mediated rejection-associated fibrogenesis in kidney transplant recipients
    Article Snippet: .. Detailed TEM8 immunofluorescence staining procedure on human kidney biopsy cryosections Fixation Acetone (-20oC) for 10 minutes Peroxidase inactivation H2O2 (1:1000 dilution, Sigma-Aldrich #216763) for 30 minutes 1st background Blocking 1%BSA in PBS for 30 minutes Primary antibody Rabbit pAb anti-human TEM8 (1:2000 dilution, Bioss #bs- 15583R) overnight at 4oC 2nd background blocking 10% normal human serum in PBS for 30 minutes Secondary antibody Goat anti-rabbit IgG HRP (1:100 dilution, DAKO #P0448) for 30 minutes + 5% normal human serum + 1%BSA Tertiary antibody Rabbit anti-goat IgG HRP (1:100 dilution, DAKO #P0449) for 30 minutes + 5% normal human serum + 1%BSA HRP Signal amplification TSA Tyramide-TRITC (1:50 dilution, Akoya Biosciences # SAT702001EA) in amplification buffer for 10 minutes Nuclear staining DAPI (Sigma-Aldrich #D9542) for 10 minutes Embedment Citifluor (Haffield PA, USA) Unless stated otherwise, the incubations were done at room temperature. ..

    Staining:

    Article Title: Urinary endotrophin as a biomarker for T cell–mediated rejection-associated fibrogenesis in kidney transplant recipients
    Article Snippet: .. Detailed TEM8 immunofluorescence staining procedure on human kidney biopsy cryosections Fixation Acetone (-20oC) for 10 minutes Peroxidase inactivation H2O2 (1:1000 dilution, Sigma-Aldrich #216763) for 30 minutes 1st background Blocking 1%BSA in PBS for 30 minutes Primary antibody Rabbit pAb anti-human TEM8 (1:2000 dilution, Bioss #bs- 15583R) overnight at 4oC 2nd background blocking 10% normal human serum in PBS for 30 minutes Secondary antibody Goat anti-rabbit IgG HRP (1:100 dilution, DAKO #P0448) for 30 minutes + 5% normal human serum + 1%BSA Tertiary antibody Rabbit anti-goat IgG HRP (1:100 dilution, DAKO #P0449) for 30 minutes + 5% normal human serum + 1%BSA HRP Signal amplification TSA Tyramide-TRITC (1:50 dilution, Akoya Biosciences # SAT702001EA) in amplification buffer for 10 minutes Nuclear staining DAPI (Sigma-Aldrich #D9542) for 10 minutes Embedment Citifluor (Haffield PA, USA) Unless stated otherwise, the incubations were done at room temperature. ..

    Blocking Assay:

    Article Title: Urinary endotrophin as a biomarker for T cell–mediated rejection-associated fibrogenesis in kidney transplant recipients
    Article Snippet: .. Detailed TEM8 immunofluorescence staining procedure on human kidney biopsy cryosections Fixation Acetone (-20oC) for 10 minutes Peroxidase inactivation H2O2 (1:1000 dilution, Sigma-Aldrich #216763) for 30 minutes 1st background Blocking 1%BSA in PBS for 30 minutes Primary antibody Rabbit pAb anti-human TEM8 (1:2000 dilution, Bioss #bs- 15583R) overnight at 4oC 2nd background blocking 10% normal human serum in PBS for 30 minutes Secondary antibody Goat anti-rabbit IgG HRP (1:100 dilution, DAKO #P0448) for 30 minutes + 5% normal human serum + 1%BSA Tertiary antibody Rabbit anti-goat IgG HRP (1:100 dilution, DAKO #P0449) for 30 minutes + 5% normal human serum + 1%BSA HRP Signal amplification TSA Tyramide-TRITC (1:50 dilution, Akoya Biosciences # SAT702001EA) in amplification buffer for 10 minutes Nuclear staining DAPI (Sigma-Aldrich #D9542) for 10 minutes Embedment Citifluor (Haffield PA, USA) Unless stated otherwise, the incubations were done at room temperature. ..

    Amplification:

    Article Title: Urinary endotrophin as a biomarker for T cell–mediated rejection-associated fibrogenesis in kidney transplant recipients
    Article Snippet: .. Detailed TEM8 immunofluorescence staining procedure on human kidney biopsy cryosections Fixation Acetone (-20oC) for 10 minutes Peroxidase inactivation H2O2 (1:1000 dilution, Sigma-Aldrich #216763) for 30 minutes 1st background Blocking 1%BSA in PBS for 30 minutes Primary antibody Rabbit pAb anti-human TEM8 (1:2000 dilution, Bioss #bs- 15583R) overnight at 4oC 2nd background blocking 10% normal human serum in PBS for 30 minutes Secondary antibody Goat anti-rabbit IgG HRP (1:100 dilution, DAKO #P0448) for 30 minutes + 5% normal human serum + 1%BSA Tertiary antibody Rabbit anti-goat IgG HRP (1:100 dilution, DAKO #P0449) for 30 minutes + 5% normal human serum + 1%BSA HRP Signal amplification TSA Tyramide-TRITC (1:50 dilution, Akoya Biosciences # SAT702001EA) in amplification buffer for 10 minutes Nuclear staining DAPI (Sigma-Aldrich #D9542) for 10 minutes Embedment Citifluor (Haffield PA, USA) Unless stated otherwise, the incubations were done at room temperature. ..



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    primary antibody rabbit pab anti human tem8  (Bioss)
    90
    Bioss primary antibody rabbit pab anti human tem8
    Primary Antibody Rabbit Pab Anti Human Tem8, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+rabbit+pab+anti+human+tem8/TEM8+Polyclonal+Antibody/pmc12596184__sfaf301_supplemental_file-26-35-43
    Average 90 stars, based on 1 article reviews
    primary antibody rabbit pab anti human tem8 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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